Join us this spring for our new Epigenomics workshops season! Our à la carte program (March 12 - April 9) is completely modular. You can join the sessions that are the most relevant for your research. Plus, get an introduction to new and upcoming technologies in epigenomics analyses.

 

All of our live sessions are highly interactive, with a mix of lecture, demos, use cases and fun activities to help you master the techniques behind chromatin and DNA methylation studies. Novice or expert, you'll learn something new with our passionate epigenomics experts covering workflows in depth – sample preparation, wetlab protocols, library preparation, analysis and bioinformatics.

 

 

 


 

- Epiworkshops program -

Each session (~ 90 min incl. Q&A) will cover the following details:

 

 

Chromatin studies


 

Chromatin immunoprecipitation (ChIP)

 

 

ChIP for plants

 

 

Tagmentation-based methods

 

 

ChIP analysis and bioinfo

 

 

9:00 PST (Los Angeles) / 12:00 EST (New York)
9:00 & 17:00 CET (Paris)

 

 

 

9:00 PST (Los Angeles) / 12:00 EST (New York)
17:00 CET (Paris)

 

 

 

9:00 & 17:00 PST (Los Angeles) / 9:00 & 12:00 EST (New York)
9:00 & 14:00 & 17:00 CET (Paris)

 

 

 

9:00 PST (Los Angeles) / 12:00 EST (New York)
17:00 CET (Paris)

 

 

ChIP overview: ChIP-qPCR or ChIP-Seq

 

 

ChIP-qPCR or ChIP-seq?

 

 

Chromatin profiling methods: ChIPmentation, CUT&Tag, ATAC-seq

 

 

ChIP-qPCR and ChIP-seq analysis

Chromatin preparation: fixation, cell lysis and chromatin shearing   Chromatin preparation: fixation, lysis and chromatin shearing   Tagmentation workflow and comparison with other methods   Bioinformatics analysis: specifics of sequencing for chromatin technologies (ChIP-Seq, ATAC-Seq, Cut&Tag)
Setting up your IP: antibodies, replicates, inputs, positive/negative controls   Setting up your IP: antibodies, replicates, inputs, positive/negative controls   iDeal CUT&Tag protocol   Sequencing QC
Library preparation and sequencing   ChIP-qPCR: set-up and analysis   Library preparation   Computer resources recommendations
    ChIP-seq: library preparation, sequencing & analysis   Analysis   Software needed
    Overview of DNA methylation methods for Plants        

 

 

DNA Methylation studies


 

DNA Methylation - Methods Landscape and how to choose

 

 

DNA Methylation analysis and bioinfo

 

 

9:00 PST (Los Angeles) / 12:00 EST (New York)
9:00 & 18:00 CET (Paris)

 

 

 

9:00 PST (Los Angeles) / 12:00 EST (New York)
18:00 CET (Paris)

 

 

What is DNA Methylation and why study it?

 

 

MeDIP-seq and MeDIP-qPCR analysis

Overview of commonly used workflows for 5mC and 5hmC analysis   RRBS analysis
Data characteristics: resolution, quality, coverage   Bioinformatics analysis: specifics of sequencing for single nucleotide resolution technologies (WGBS, RRBS, Targeted)
Applicability to species and sample types   Sequencing QC
 

Workflows, tips & tricks for selected methods - Which one should I choose?

  •   • Methylated DNA Immunoprecipitation (MeDIP) - Genome-wide and cost effective
  •   • WGBS and RRBS - Your DNA, sheared or digested?
  •   • Infinium Arrays, Human Methylome Panel - Capture targeted methods
  Computer resources recommendations
    Software needed
     

 

 

 

Select your session(s), save your spot and register now!