Join us this fall for our new Epigenomics workshops season! Our à la carte program (October 22-29 & November 5-12-19-26) is completely modular. You can join the sessions that are the most relevant for your research. Plus, get an introduction to new and upcoming technologies in epigenomics analyses.
Each workshop will be offered several times throughout the day, as outlined in the schedule.
All of our live sessions are highly interactive, with a mix of lecture, demos, use cases and fun activities to help you master the techniques behind chromatin and DNA methylation studies. Novice or expert, you'll learn something new with our passionate epigenomics experts covering workflows in depth – sample preparation, wetlab protocols, library preparation, analysis and bioinformatics.
October 22: Chromatin
Paris: 9:00am & 18:00pm
New York: 12:00pm & 18:00pm
Los Angeles: 9:00am & 15:00pm
October 29: Tagmentation
Paris: 9:00am & 17:00pm
New York: 12:00pm & 18:00pm
Los Angeles: 9:00am & 15:00pm
November 5: Bioinfo for Chromatin studies
Paris: 18:00pm
New York: 12:00pm
Los Angeles: 9:00am
November 12: DNA methylation
Paris: 9:00am & 18:00pm
New York: 12:00pm & 18:00pm
Los Angeles: 9:00am & 15:00pm
November 19: Bioinfo for DNA methylation
Paris: 18:00pm
New York: 12:00pm
Los Angeles: 9:00am
November 26: RNA-seq
Paris: 9:00am & 18:00pm
New York: 12:00pm & 18:00pm
Los Angeles: 9:00am & 15:00pm
| 22 Oct – ChIP | 29 Oct – Tagmentation | 5 Nov – Bioinfo for Chromatin studies |
| ChIP overview: ChIP-qPCR or ChIP-seq | Chromatin profiling methods: ChIPmentation, CUT&Tag, ATAC-seq | ChIP-qPCR and ChIP-seq analysis |
| Chromatin preparation: fixation, cell lysis and chromatin shearing | Tn5 transposase, a world of applications | Bioinformatics analysis: specifics of sequencing for chromatin technologies (ChIP-Seq, ATAC-Seq, Cut&Tag) |
| Setting up your IP: antibodies, replicates, inputs, positive/negative controls | Tagmentation workflow and comparison with other methods | Sequencing QC |
| Library preparation and sequencing | iDeal CUT&Tag protocol | Computer resources recommendations |
| Method considerations for Plant samples | ATAC-seq protocol | Software needed |
| Case study | Case study | |
| Duration: 120 min | Duration: 120 min | Duration: 90 min |
| 12 Nov – DNA methylation | 19 Nov – Bioinfo for DNA methylation | 26 Nov – RNA-seq |
| Overview of commonly used workflows for 5mC and 5hmC analysis | Specifics of sequencing for single nucleotide resolution technologies (WGBS, RRBS, Targeted) | Introduction on non-coding RNA |
| Output characteristics: resolution, quality, coverage - a comparison | Sequencing QC | Overview of common RNA-seq analyses |
| Biomarker discovery pipeline -which method to choose? | Computer resources and software recommendations | Our innovative take on RNA-seq: the D-Plex technology |
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Workflows, tips & tricks for selected methods: • MeDIP-qPCR/-seq • WGBS, EM-seq and RRBS • Infinium Arrays, Human Methylome Panel |
Step-by-step workflow and example of results | |
| Case study | A World of applications: from ribosome profiling to biomarker discovery | |
| Case study | ||
| Duration: 120 min | Duration: 90 min | Duration: 90 min |
Hologic Diagenode is your dedicated supplier for epigenomics profiling services and sample preparation products for next generation sequencing, proteomics, and epigenetics research. Our experimentally-validated, easy-to-use solutions and services for RNA, DNA, chromatin, and protein analysis give researchers the confidence and success needed to achieve best-in-class results.
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