October 21-28, November 18-25 & December 2
Join us this fall for our new Epigenomics workshops season! Our à la carte program is completely modular. You can join the sessions that are the most relevant for your research. Plus, get an introduction to new and upcoming technologies in epigenomics analyses.
Each workshop will be offered several times throughout the day, as outlined in the schedule.
All of our live sessions are highly interactive, with a mix of lecture, demos, use cases and fun activities to help you master the techniques behind chromatin and DNA methylation studies. Novice or expert, you'll learn something new with our passionate epigenomics experts covering workflows in depth – sample preparation, wetlab protocols, library preparation, analysis and bioinformatics.
ChIP overview: ChIP-qPCR or ChIP-seq
Chromatin preparation: fixation, cell lysis and chromatin shearing
Setting up your IP: antibodies, replicates, inputs, positive/negative controls
Library preparation and sequencing
Method considerations for Plant samples
Case study
Chromatin profiling methods: ChIPmentation, CUT&Tag, ATAC-seq
Tn5 transposase, a world of applications
Tagmentation workflow and comparison with other methods
iDeal CUT&Tag protocol
ATAC-seq protocol
Case study
ChIP-qPCR and ChIP-seq analysis
Bioinformatics analysis: specifics of sequencing for chromatin technologies (ChIP-seq, ATAC-seq, CUT&Tag)
Sequencing QC
Computer resources recommendations
Software needed
Overview of commonly used workflows for 5mC and 5hmC analysis
Output characteristics: resolution, quality, coverage - a comparison
Biomarker discovery pipeline - which method to choose?
Workflows, tips & tricks for selected methods
- MeDIP-qPCR/-seq
- WGBS, EM-seq and RRBS
- Infinium Arrays, Human Methylome Panel
Case study
Specifics of sequencing for single nucleotide resolution technologies (WGBS, RRBS, Targeted)
Sequencing QC
Computer resources and software recommendations
Library preparation and sequencing
Introduction on non-coding RNA
Overview of common RNA-seq analyses
Our innovative take on RNA-seq: the D-Plex technology
Step-by-step workflow and example of results
A World of applications: from ribosome profiling to biomarker discovery
Case study
Hologic Diagenode is your dedicated supplier for epigenomics profiling services and sample preparation products for next generation sequencing, proteomics, and epigenetics research. Our experimentally-validated, easy-to-use solutions and services for RNA, DNA, chromatin, and protein analysis give researchers the confidence and success needed to achieve best-in-class results.
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