Join us this spring for our new Epigenomics workshops season! Our à la carte program (March 11-18-25 & April 1-8-15) is completely modular. You can join the sessions that are the most relevant for your research. Plus, get an introduction to new and upcoming technologies in epigenomics analyses.
Each workshop will be offered several times throughout the day, as outlined in the schedule.
All of our live sessions are highly interactive, with a mix of lecture, demos, use cases and fun activities to help you master the techniques behind chromatin and DNA methylation studies. Novice or expert, you'll learn something new with our passionate epigenomics experts covering workflows in depth – sample preparation, wetlab protocols, library preparation, analysis and bioinformatics.
March 11: Chromatin
Paris: 9:00 & 17:00
New York: 12:00 & 16:00
Los Angeles: 9:00 & 13:00
March 18: Tagmentation
Paris: 9:00 & 17:00
New York: 12:00 & 16:00
Los Angeles: 9:00 & 13:00
March 25: Bioinfo for Chromatin studies
Paris: 17:00
New York: 12:00
Los Angeles: 9:00
April 1: DNA methylation
Paris: 9:00 & 18:00
New York: 12:00 & 16:00
Los Angeles: 9:00 & 13:00
April 8: Bioinfo for DNA methylation
Paris: 18:00
New York: 12:00
Los Angeles: 9:00
April 15: RNA-seq
Paris: 9:00 & 18:00
New York: 12:00 & 16:00
Los Angeles: 9:00 & 13:00
| 11 Mar – ChIP | 18 Mar – Tagmentation | 25 Mar – Bioinfo for Chromatin studies |
| ChIP overview: ChIP-qPCR or ChIP-seq | Chromatin profiling methods: ChIPmentation, CUT&Tag, ATAC-seq | ChIP-qPCR and ChIP-seq analysis |
| Chromatin preparation: fixation, cell lysis and chromatin shearing | Tn5 transposase, a world of applications | Bioinformatics analysis: specifics of sequencing for chromatin technologies (ChIP-Seq, ATAC-Seq, Cut&Tag) |
| Setting up your IP: antibodies, replicates, inputs, positive/negative controls | Tagmentation workflow and comparison with other methods | Sequencing QC |
| Library preparation and sequencing | iDeal CUT&Tag protocol | Computer resources recommendations |
| Method considerations for Plant samples | ATAC-seq protocol | Software needed |
| Case study | Case study | |
| Duration: 120 min | Duration: 120 min | Duration: 90 min |
| 1 Apr – DNA methylation | 8 Apr – Bioinfo for DNA methylation | 15 Apr – RNA-seq |
| Overview of commonly used workflows for 5mC and 5hmC analysis | Specifics of sequencing for single nucleotide resolution technologies (WGBS, RRBS, Targeted) | Introduction on non-coding RNA |
| Output characteristics: resolution, quality, coverage - a comparison | Sequencing QC | Overview of common RNA-seq analyses |
| Biomarker discovery pipeline -which method to choose? | Computer resources and software recommendations | Our innovative take on RNA-seq: the D-Plex technology |
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Workflows, tips & tricks for selected methods: • MeDIP-qPCR/-seq • WGBS, EM-seq and RRBS • Infinium Arrays, Human Methylome Panel |
Step-by-step workflow and example of results | |
| Case study | A World of applications: from ribosome profiling to biomarker discovery | |
| Case study | ||
| Duration: 120 min | Duration: 90 min | Duration: 90 min |
Hologic Diagenode is your dedicated supplier for epigenomics profiling services and sample preparation products for next generation sequencing, proteomics, and epigenetics research. Our experimentally-validated, easy-to-use solutions and services for RNA, DNA, chromatin, and protein analysis give researchers the confidence and success needed to achieve best-in-class results.
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